A substoicheiometric assay for the determination of free adenosine triphosphate in the presence of reversibly bound adenosine triphosphate

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The Eq+librium Constants of the Adenosine Triphosphate Hydrolysis and the Adenosine Triphosphate- Citrate Lyase Reactions

The observed standard free energy change (AGibs) for the hydrolysis of the terminal pyrophosphate bond of ATP has been experimentally determined under physiological conditions using an entirely new set of reactions. The observed equilibrium constant (K,,bs) for the combined reactions of acetate kinase (EC 2.7.2.1) and phosphate acetyltransferase (EC 2.3.1.8) has been determined at 38”, pH 7.0, ...

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The equilibrium constants of the adenosine triphosphate hydrolysis and the adenosine triphosphate-citrate lyase reactions.

The observed standard free energy change (AGibs) for the hydrolysis of the terminal pyrophosphate bond of ATP has been experimentally determined under physiological conditions using an entirely new set of reactions. The observed equilibrium constant (K,,bs) for the combined reactions of acetate kinase (EC 2.7.2.1) and phosphate acetyltransferase (EC 2.3.1.8) has been determined at 38”, pH 7.0, ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1970

ISSN: 0306-3283

DOI: 10.1042/bj1160039pb